edaravone decreases paraquat toxicity in a549 cells and lung isolated mitochondria

نویسندگان

mohammad shokrzadeh pharmaceutical research center, department of toxicology and pharmacology, faculty of pharmacy, mazandaran university of medical sciences, sari, iran.

fatemeh shaki

ebrahim mohammadi department of toxicology and pharmacology, faculty of pharmacy, shahid beheshti university of medical sciences, tehran, iran.

neda rezagholizadeh pharmaceutical research center, department of toxicology and pharmacology, faculty of pharmacy, mazandaran university of medical sciences, sari, iran

چکیده

edaravone, an antioxidant and radical scavenger, showed protective effects against oxidative stress-like condition. paraquat (pq) is a toxic herbicide considerable evidence suggests that oxidative stress and mitochondrial dysfunction contribute to pq toxicity. in this study, protective effect of edaravone against pq induced toxicity and reactive oxygen species (ros) generation in a549 cells and lung isolated mitochondria were evaluated. a549 cells and lung isolated mitochondria were divided into control group, pq group, edaravone group and pq plus edaravone-pretreated group. cellular and mitochondrial viability assayed using mtt test and ros generations in both cellular and mitochondrial fraction were determined by fluorometry using dcfh-da as indicator.our results showed that edaravone (5–100 µm) prevented pq (500 µm ) induced cytotoxicity in a549 cells that the best protective effect was observed at concentration of 50 µm of edaravone. in addition, pq-induced ros generation in a549 cells significantly inhibited by edaravone. moreover, pq decreased mitochondria viability and also increased ros generation in lung isolated mitochondria that edaravone (25–400 µm) markedly inhibited these toxic effects. in overall, the results of this study suggest that lung mitochondria maintenance is essential for maintaining pqt cytotoxicity and edaravone is a protective drug against pq toxicity in vitro.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Edaravone Decreases Paraquat Toxicity in A549 Cells and Lung Isolated Mitochondria

Edaravone, an antioxidant and radical scavenger, showed protective effects against oxidative stress-like condition. Paraquat (PQ) is a toxic herbicide considerable evidence suggests that oxidative stress and mitochondrial dysfunction contribute to PQ toxicity. In this study, protective effect of edaravone against PQ induced toxicity and reactive oxygen species (ROS) generation in A549 cells and...

متن کامل

Edaravone Decreases Paraquat Toxicity in A549 Cells and Lung Isolated Mitochondria

Edaravone, an antioxidant and radical scavenger, showed protective effects against oxidative stress-like condition. Paraquat (PQ) is a toxic herbicide considerable evidence suggests that oxidative stress and mitochondrial dysfunction contribute to PQ toxicity. In this study, protective effect of edaravone against PQ induced toxicity and reactive oxygen species (ROS) generation in A549 cells and...

متن کامل

Edaravone Decreases Paraquat Toxicity in A549 Cells and Lung Isolated Mitochondria

Edaravone, an antioxidant and radical scavenger, showed protective effects against oxidative stress-like condition. Paraquat (PQ) is toxic herbicide considerable evidence suggests that oxidative stress and mitochondrial dysfunction contribute to PQ toxicity. In this study, protective effect of edaravone against PQ induced toxicity and reactive oxygen species (ROS) generation in A549 cells and l...

متن کامل

Toxicity of atorvastatin on isolated brain mitochondria

Background: Although the bio kinetics, metabolism and chemical toxicity of Atorvastatin are well known, until recently little attention was paid to the potential neurotoxic effect of Atorvastatin (Atv). Regarding the concrete evidences indicating Atv may reduce Coenzyme Q10 (CoQ10) levels through blockage of  metalonate cycle, the present work aims to determine if Atorvastatin may provide toxic...

متن کامل

Captopril ameliorates toxicity induced by paraquat in mitochondria isolated from the rat liver.

The aim of the present study was to show the abilities of captopril as a thiol ACEi (angiotensin converting enzyme inhibitor), on mitochondria toxicity due to paraquat. Mitochondrial isolation from rat liver was divided into 4 groups. Group 1 was considered as control, group 2 received paraquat (5 mM), group 3 received captopril (0.08 mM) and group 4 received paraquat (5 mM)+captopril (0.08 mM)...

متن کامل

the ameliorative effect of naringenin on paraquat-induced toxicity in mitochondria isolated from rats

results the results reveal that pq can decrease mitochondrial viability, deplete gsh content, increase mitochondrial ros formation, and enhance lpo and mitochondrial membrane damage. the pretreatment of mitochondria with ng has the ability to reduce the toxic effects of pq in isolated mitochondria. background paraquat (pq) is a dipyridyl herbicide that sustains damage due to redox cycling. an i...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۳، شماره ۲، صفحات ۶۷۵-۶۸۱

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023